Surface modified single molecules free-diffusion evidenced by fluorescence correlation spectroscopy
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Updated: Jul 6, 2026

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Published on: November 12, 2020
Céline Boutin1, Rodolphe Jaffiol, Jérôme Plain
1Laboratoire de Nanotechnologie et d'Instrumentation Optique, LRC CEA, ICD CNRS FRE2848, Université de Technologie de Troyes, 12 rue Marie Curie, BP2060, 10010, Troyes cedex, France.
Abstract:
We report on the free diffusion of single molecule near an interface studied using fluorescence correlation spectroscopy. In particular, we show that the chemical nature of the substrate may modify the free diffusion of a widely used molecule (rhodamine 6G), thus inducing unexpected effects in fluorescence correlation spectroscopy measurements. Our results show a strong influence, up to a few micrometer from the interface, of the surface polarity. This effect is assessed through the relative weight of the two dimensions diffusion process observed close to the surface. Our results are discussed in terms of competition between surface-solvent, solvent-molecule and molecule-surface specific interactions.
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