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Related Experiment Video

Updated: Jul 6, 2026

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
08:51

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

Published on: May 13, 2016

Intercalibration exercise using a stickleback endocrine disrupter screening assay.

Yvonne T Allen1, Ioanna Katsiadaki, Tom G Pottinger

  • 1Centre for Environment, Fisheries, and Aquaculture Science, Burnham Laboratory, Burnham-on-Crouch, Essex, UK. yvonne.allen@cefas.co.uk

Environmental Toxicology and Chemistry
|March 20, 2008
PubMed
Summary
This summary is machine-generated.

The three-spined stickleback fish assay reliably detects potent estrogens and androgens. This endocrine disruptor screening method shows promise for inclusion in international environmental safety protocols.

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Last Updated: Jul 6, 2026

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Area of Science:

  • Environmental Toxicology
  • Endocrinology
  • Aquatic Ecotoxicology

Background:

  • The Organisation for Economic Cooperation and Development (OECD) is validating fish screening protocols for endocrine-disrupting chemicals.
  • Key endpoints include vitellogenin (VTG), morphology, and gonadal histopathology.
  • A similar protocol using the three-spined stickleback is under development, including a unique spiggin endpoint.

Purpose of the Study:

  • To assess the suitability of the three-spined stickleback for inclusion in the OECD endocrine disruptor screening protocol.
  • To intercalibrate the stickleback assay with core OECD species using known estrogen and androgen compounds.

Main Methods:

  • An intercalibration study was conducted using 17beta-estradiol (estrogen) and trenbolone (androgen).
  • Three laboratories exposed sticklebacks to these compounds for 14 days.
  • Measured endpoints included vitellogenin (VTG) in males and spiggin in females.

Main Results:

  • Statistically significant increases in VTG were detected in male sticklebacks exposed to 17beta-estradiol.
  • Statistically significant increases in spiggin were detected in female sticklebacks exposed to trenbolone.
  • The assay demonstrated reliability and reproducibility across participating laboratories.

Conclusions:

  • The three-spined stickleback screening assay is a reliable method for detecting potent estrogens and androgens.
  • The protocol possesses relevant and reproducible endpoints.
  • Further research will evaluate its efficacy for weakly acting compounds and other endocrine disruptors.