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Fas expression on peritoneal macrophages during continuous ambulatory peritoneal dialysis peritonitis
Chen-Yin Chen1, Chan-Yao Wu, Tsung-Chang Tsai
1Division of Nephrology, Department of Medicine, Tian-Sheng Memorial Hospital, Tong-Kang, Ping-Tong, Taiwan.
Background:
Peritonitis is a common complication of end stage renal failure (ESRF) patients receiving continuous ambulatory peritoneal dialysis (CAPD). Peritoneal macrophage may participate in the activation of specific T cells and in the generation of local cell-mediated immunity to various pathogens. The purpose of this study is to investigate the possible role of macrophage in CAPD patients with peritonitis.
Methods:
We evaluated the expression of Fas receptor (CD95), ICAM-1 (CD54), CD25, and CD69 by two-color flow cytometry on extravasted macrophages from 16 ESRF patients on CAPD with peritonitis (peritonitis-positive) and compared them to 11 ESRF patients on CAPD without peritonitis (peritonitis-negative) and normal controls.
Results:
We found an increased expression of CD95, CD54, and CD25 on macrophage in peritonitis-positive group compared to controls (all p < 0.001). In the peritonitis-positive group, the CD95 expression was significantly higher than that of the peritonitis-negative group (p < 0.001). The expression of CD54, CD25, and CD69, however, was not significantly different between the peritonitis-positive and peritonitis-negative CAPD subgroups.
Conclusion:
We found an abnormally increased percentage of macrophage-expressing Fas receptor and ICAM-1, and the percentage of CD95+ macrophage, but not those of other markers, were increased among the subset of CAPD patients with peritonitis. The later finding suggests that this macrophage phenotype is associated with peritonitis occurring in CAPD.
Insights
Macrophages expressing Fas receptor (CD95) and ICAM-1 are increased in patients with end-stage renal failure on continuous ambulatory peritoneal dialysis (CAPD) who develop peritonitis. This specific macrophage phenotype is associated with CAPD peritonitis.
Area of Science:
- Nephrology
- Immunology
- Cell Biology
Background:
- Peritonitis is a frequent complication for end-stage renal failure (ESRF) patients undergoing continuous ambulatory peritoneal dialysis (CAPD).
- Peritoneal macrophages play a role in immune responses against pathogens.
Purpose of the Study:
- To investigate the role of macrophages in CAPD patients experiencing peritonitis.
Main Methods:
- Two-color flow cytometry was used to evaluate macrophage markers (Fas receptor/CD95, ICAM-1/CD54, CD25, CD69).
- Macrophages were analyzed from ESRF patients on CAPD with (peritonitis-positive) and without (peritonitis-negative) peritonitis, along with normal controls.
Main Results:
- Increased expression of CD95, CD54, and CD25 was observed on macrophages in the peritonitis-positive group compared to controls (p < 0.001).
- CD95 expression was significantly higher in the peritonitis-positive group versus the peritonitis-negative group (p < 0.001).
- No significant differences in CD54, CD25, or CD69 expression were found between the peritonitis-positive and peritonitis-negative CAPD subgroups.
Conclusions:
- An elevated percentage of macrophages expressing Fas receptor (CD95) and ICAM-1 was identified in CAPD patients with peritonitis.
- The increased CD95+ macrophage subset is specifically associated with peritonitis in CAPD patients.
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