Related Experiment Video

Updated: Jul 6, 2026

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
12:00

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

Published on: July 5, 2017

Apple polyphenols modulate expression of selected genes related to toxicological defence and stress response in human

Selvaraju Veeriah1, Claudia Miene, Nina Habermann

  • 1Department of Nutritional Toxicology, Institute for Nutrition, Friedrich-Schiller-University, Jena, Germany.

International Journal of Cancer
|March 21, 2008
PubMed

Insights

Apple extracts (AEs) containing polyphenols significantly altered gene expression in preneoplastic colon cells. This suggests AEs may offer protection against colon cancer by modulating key genes involved in chemoprevention.

Area of Science:

  • Nutritional Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • Apples possess flavonoids with potential antioxidative and antiproliferative properties.
  • These compounds may reduce cancer risk by favorably modulating gene expression.

Purpose of the Study:

  • To investigate apple polyphenol effects on gene expression related to colon cancer prevention.
  • To analyze modulation of genes in preneoplastic colon adenoma cells (LT97).

Main Methods:

  • LT97 cells treated with apple extracts (AEs).
  • Gene expression analyzed using commercial (96-gene) and custom (300-gene) cDNA microarrays.
  • Real-time PCR and enzyme activity assays confirmed array results.

Main Results:

  • AE treatment altered expression of 30 and 46 genes in the Superarray and custom array, respectively.
  • Four genes (CYP3A7, CYP4F3, CHST7, GSTT2) showed consistent directional changes on both arrays.
  • Expression of key phase II genes (e.g., GSTP1, UGT1A1) and enzyme activities (GST, UGT) were modulated.

Conclusions:

  • Apple extracts significantly alter gene expression patterns in preneoplastic colon cells.
  • Modulated gene expression suggests a potential protective role of apple polyphenols against toxicological insults.
  • Findings support the chemopreventive potential of apple-derived compounds in colon cancer.

More Related Videos

A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals07:39

A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

Published on: June 25, 2018

Related Concept Videos

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...