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Dose-dependent stimulation of bone induction by basic fibroblast growth factor in rats
P Aspenberg1, K G Thorngren, L S Lohmander
1Lund University Hospital Department of Orthopedics, Sweden.
Acta Orthopaedica Scandinavica
|October 1, 1991
Summary
Recombinant human basic fibroblast growth factor (bFGF) enhances new bone formation with demineralized bone matrix implants. Optimal bone yield occurred at specific bFGF doses, highlighting its complex role in bone regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Orthopedics
Background:
- Demineralized bone matrix (DBM) implantation in rodents stimulates new bone formation.
- Bone morphogenetic proteins and local growth factors are key regulators of this osteogenic process.
- Previous studies indicated recombinant human basic fibroblast growth factor (bFGF) can increase bone yield.
Purpose of the Study:
- To further investigate the temporal effects of bFGF on bone formation stimulated by DBM.
- To determine the dose-dependent efficacy and optimal dosage range of bFGF for bone regeneration.
Main Methods:
- Surgical implantation of DBM with varying doses of bFGF in a carboxymethyl cellulose gel in a rat model.
- Quantification of new bone formation by measuring calcium content at multiple time points post-implantation.
Main Results:
- A significant increase in bone yield was observed at 3 and 4 weeks post-implantation with bFGF.
- Stimulatory effects were noted for bFGF doses ranging from 3 to 75 ng per implant.
- Lower (0.6, 380 ng) and higher (1,900 ng) doses of bFGF showed no enhancement or significant inhibition, respectively.
Conclusions:
- Local application of bFGF can enhance bone regeneration mediated by DBM implants.
- The osteogenic effect of bFGF is dose-dependent, with a narrow optimal window for efficacy.
- The complex, dose-sensitive actions of bFGF warrant further investigation for clinical applications in bone repair.