Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inflammatory gene changes associated with the repeated-bout effect.

Monica J Hubal1, Trevor C Chen, Paul D Thompson

  • 1Department of Kinesiology, University of Massachusetts, Amherst, MA, USA. mhubal@cnmcresearch.org

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
|March 21, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulation of Small RNAs by Exercise and Their Role in Insulin Sensitivity.

bioRxiv : the preprint server for biology·2026
Same author

The Acute Effect of Resistance Exercise in Individuals With Hypertrophic Cardiomyopathy: A Pilot Study.

The American journal of cardiology·2026
Same author

Prevalence and Predictors of Cardiac Troponin Elevations Following Exercise: a Systematic Review, Meta-analysis, and Meta-regression.

European journal of preventive cardiology·2026
Same author

Sudden Cardiac Arrest in Athletes.

The New England journal of medicine·2026
Same author

Masters athletes with abnormal cardiovascular findings: a clinical consensus statement of the European Association of Preventive Cardiology of the ESC and the American College of Cardiology.

European heart journal·2026
Same author

Masters Athletes With Abnormal Cardiovascular Findings: A Clinical Consensus Statement of the European Association of Preventive Cardiology of the ESC and the American College of Cardiology.

Journal of the American College of Cardiology·2026
Same journal

Changes in IMCL content and storage characteristics during an acute bout of high intensity interval exercise are influenced by sex and muscle fibre type.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
Same journal

The Cardiorespiratory Response of Atlantic Salmon and Nile Tilapia to Similar Acute Thermal and Hypoxic Challenges: The Complete Story.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
Same journal

Early Life Stress Blunts Time Of Day Specific Autonomic Function In Adult Male Mice.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
Same journal

Homeostatic adaptations of the amphibious fish <i>Polypterus</i> in terrestrial environments.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
Same journal

Renal Hemodynamic and Urinary Response to Baroreflex-Mediated Sympathetic Modulation: Insights from High-Resolution Arterial Impedance Analysis in Rats.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
Same journal

Gravitational load, sex, and age-sex effects on cardiovascular compensatory responses during sit-to-stand and lie-to-stand transitions.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
See all related articles

Repeated exercise bouts enhance muscle adaptation. Inflammatory gene expression, particularly ZFP36, CEBPD, and monocyte chemoattractant protein-1 (MCP1), was upregulated after the second bout, suggesting a role in this adaptation.

Area of Science:

  • Exercise physiology
  • Molecular biology
  • Muscle adaptation

Background:

  • The repeated-bout effect describes rapid adaptation to exercise after prior exposure.
  • Inflammatory factors were hypothesized to be attenuated, contributing to this adaptation.

Purpose of the Study:

  • To investigate changes in inflammatory gene mRNA and protein after two bouts of muscle-lengthening exercise.
  • To explore the role of inflammatory gene expression in the repeated-bout effect.

Main Methods:

  • Seven male subjects performed two bouts of eccentric-concentric or concentric-only leg exercise.
  • Vastus lateralis muscle biopsies and strength assessments were conducted.
  • mRNA levels of eight inflammatory genes (CYR61, HSP40, HSP70, IL1R1, TCF8, ZFP36, CEBPD, MCP1) were measured via RT-PCR.

Related Experiment Videos

  • Immunohistochemistry localized monocyte chemoattractant protein-1 (MCP1) protein.

    • Main Results:

    • Eccentrically exercised legs showed attenuated strength loss (16% less) after bout 2 compared to bout 1.
    • mRNA expression of ZFP36, CEBPD, and MCP1 significantly increased in the eccentrically exercised leg after bout 2.
    • No changes in mRNA levels were observed in the concentrically exercised leg.
    • MCP1 protein colocalized with macrophages and satellite cells.

    Conclusions:

    • Contrary to the hypothesis, inflammatory genes were upregulated, not attenuated, after a repeated exercise bout.
    • Upregulated inflammatory gene expression, particularly MCP1, may play a role in muscle adaptation to exercise.
    • Cytokine signaling between macrophages and satellite cells could be involved in exercise adaptation.