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Related Experiment Video

Updated: Jul 6, 2026

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Published on: May 3, 2011

Interferometric sensor for detection of surface-bound bioreactions.

A Brandenburg¹, R Krauter, C Künzel

¹Fraunhofer-Institute of Physical Measurement Techniques, Heidenhohstrasse 8, Freiburg D-79110, Germany. albrecht.brandenburg@ipm.fhg.de

|March 21, 2008

Summary

This study presents an integrated optical interferometer for detecting molecular binding. The device uses waveguide structures to achieve high sensitivity, detecting as little as 750 fg/mm² of adsorbed molecules.

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Area of Science:

Optoelectronics
Biosensing
Interferometry

Background:

Optical interferometers are crucial for precise measurements.
Existing methods for detecting affinity reactions can be complex and lack sensitivity.
Integrated optical devices offer miniaturization and enhanced performance.

Purpose of the Study:

To develop an integrated optical interferometer for direct detection of affinity reactions.
To utilize waveguide structures as both beam splitters and sensing elements.
To achieve high sensitivity and resolution in molecular adsorption detection.

Main Methods:

Fabrication of resistive waveguides using plasma-enhanced chemical vapor deposition of silicon oxinitride.
Construction of a modern Young's interferometer incorporating the waveguide structure.
Detection of fringe patterns at the output using a CCD line camera.

Main Results:

Demonstrated direct detection of molecular adsorption on waveguide surfaces.
Achieved a detection limit of 750 fg/mm² for adsorbed molecules.
Resolved variations in effective refractive index as small as 9 x 10⁻⁸.

Conclusions:

The integrated optical interferometer enables sensitive and direct detection of affinity reactions.
Waveguide-based interferometry is a promising approach for label-free biosensing.
The developed device offers high resolution for monitoring molecular interactions.