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Updated: Jul 6, 2026

Quantification of Coenzyme A in Cells and Tissues
Published on: September 27, 2019
Novel isolation procedure for short-, medium-, and long-chain acyl-coenzyme A esters from tissue
Paul E Minkler1, Janos Kerner, Stephen T Ingalls
1Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
Abstract:
A novel procedure for the quantitative isolation and purification of acyl-coenzyme A esters is presented. The procedure involves two steps: (1) tissue extraction using acetonitrile/2-propanol (3+1, v+v) followed by 0.1M potassium phosphate, pH 6.7, and (2) purification using 2-(2-pyridyl)ethyl-functionalized silica gel. Recoveries determined by adding radiolabeled acetyl-, malonyl-, octanoyl-, oleoyl-, palmitoyl-, or arachidonyl-coenzyme A to powdered rat liver varied 93-104% for tissue extraction and 83-90% for solid-phase extraction. The procedure described allows for isolation and purification, with high recoveries, of acyl-coenzyme A esters differing widely in chain length and saturation.
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