Chemically modified oligonucleotides with efficient RNase H response
Birte Vester1, Anne Marie Boel, Sune Lobedanz
1Nucleic Acid Center, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
Bioorganic & Medicinal Chemistry Letters
|March 22, 2008
Abstract:
Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.
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