Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.6K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA-targeting Invader probes: discovery, principles and applications.

RSC chemical biology·2026
Same author

An electrophoretic mobility shift assay with chemiluminescent readout to evaluate DNA-targeting oligonucleotide-based probes.

PloS one·2025
Same author

Conformation matters: siRNAs with antisense strands with 5'-(<i>E</i>)-vinyl-phosphonate-α-L-LNA elicit stronger RNAi-mediated gene silencing than those with 5'-(<i>E</i>)-vinyl-phosphonate-LNA.

Chemical communications (Cambridge, England)·2024
Same author

Recognition of mixed-sequence double-stranded DNA regions using chimeric Invader/LNA probes.

Organic & biomolecular chemistry·2024
Same author

Serine-γPNA, Invader probes, and chimeras thereof: three probe chemistries that enable sequence-unrestricted recognition of double-stranded DNA.

Organic & biomolecular chemistry·2022
Same author

Nicked Invader probes: multistranded and sequence-unrestricted recognition of double-stranded DNA.

Organic & biomolecular chemistry·2021

Related Experiment Video

Updated: Jan 14, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.5K

Efficient and specific DNA-targeting using single-stranded LNA/MOE mixmers and chimeric Invader:XenoRNA probes.

Michaela E Everly1, Patrick J Hrdlicka1

  • 1Department of Chemistry, University of Idaho, Moscow, Idaho 83844-2343, USA. hrdlicka@uidaho.edu.

Organic & Biomolecular Chemistry
|October 20, 2025
PubMed
Summary

Intercalator-functionalized oligonucleotides form heteroduplexes with Xeno RNAs, enabling efficient DNA target invasion. These DNA-targeting probes demonstrate exceptional binding specificity for double-stranded DNA.

More Related Videos

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

17.0K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.8K

Related Experiment Videos

Last Updated: Jan 14, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.5K
Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

17.0K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oligonucleotide Chemistry

Background:

  • Oligonucleotides are short DNA or RNA molecules.
  • Xeno nucleic acids (XNA) are nucleic acid analogues with modified sugar-backbone structures.
  • Intercalator-functionalized oligonucleotides can bind to DNA and RNA.

Purpose of the Study:

  • To investigate the DNA-targeting properties of heteroduplexes formed between intercalator-functionalized oligonucleotides and various Xeno RNAs.
  • To evaluate the efficiency and specificity of these DNA-targeting probes for double-stranded DNA targets.

Main Methods:

  • Synthesis of intercalator-functionalized oligonucleotides.
  • Preparation of Xeno RNA analogues including LNA/MOE mixmers, MOE, O2'-Me, and 2'-F strands.
  • Formation of heteroduplexes between oligonucleotides and Xeno RNAs.
  • Assay of DNA target invasion by the heteroduplexes.
  • Assessment of binding specificity to double-stranded DNA.

Main Results:

  • Heteroduplexes formed with LNA/MOE mixmers and other Xeno RNAs exhibit DNA-targeting properties.
  • Chimeric Invader:LNA/MOE probes and single-stranded LNA/MOE mixmers efficiently invade double-stranded DNA targets.
  • Exceptional binding specificity was observed for these DNA-targeting probes.

Conclusions:

  • Intercalator-functionalized oligonucleotide-Xeno RNA heteroduplexes are effective DNA-targeting agents.
  • LNA/MOE mixmers, particularly in chimeric and single-stranded formats, offer a promising platform for specific double-stranded DNA targeting.