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An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
Published on: November 1, 2018
Morphological changes in the tubulointerstitial compartment in primary glomerulopathies.
S Kostadinova-Kunovska1, G Petrusevska, R Jovanovic
1Institute of Pathology, Faculty of Medicine, Skopje, R. Macedonia. skkunovska@yahoo.com
Prilozi
|March 22, 2008
Summary
Epithelial-mesenchymal transition in renal tubulointerstitial cells is linked to kidney dysfunction in primary glomerulopathies. These changes, including interstitial fibrosis, correlate with serum creatinine levels, impacting nephropathological diagnosis.
Area of Science:
- Nephrology
- Pathology
- Cell Biology
Background:
- The renal interstitium plays a crucial role in kidney function and is implicated in renal dysfunction.
- Tubulointerstitial changes are common in glomerular diseases and can lead to kidney failure.
- Epithelial-mesenchymal transition (EMT) is a cellular process observed in fibrotic kidney diseases.
Purpose of the Study:
- To investigate the occurrence and significance of EMT in the tubulointerstitial compartment of renal biopsies from patients with primary glomerulopathies.
- To correlate tubulointerstitial changes with markers of renal function, such as serum creatinine.
Main Methods:
- Histochemical, immunohistochemical, and morphometric analyses of 50 renal biopsies.
- Light microscopy of semi-thin sections and double immunohistochemical stainings.
- Correlation of pathological findings with clinical data on renal function.
Main Results:
- Atrophic tubular epithelial cells showed loss of epithelial markers (Cytokeratin, E-cadherin) and gain of mesenchymal markers (Vimentin, alphaSMA), indicating EMT.
- Interstitial fibrosis was observed in 98% of biopsies.
- Tubulointerstitial changes correlated significantly with serum creatinine levels.
Conclusions:
- EMT is a key phenomenon in the tubulointerstitial compartment of primary glomerulopathies, contributing to renal dysfunction.
- Histomorphometrical and immunohistochemical analyses are valuable tools for nephropathological diagnosis.
- The study highlights the link between tubulointerstitial pathology and overall kidney function.
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