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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
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Rhodamine-based fluorogenic probe for imaging biological thiol.

Aya Shibata1, Kazuhiro Furukawa, Hiroshi Abe

  • 1Nano Medical Engineering Laboratory Discovery Research Institute, RIKEN 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan.

Bioorganic & Medicinal Chemistry Letters
|March 25, 2008
PubMed
Summary
This summary is machine-generated.

Researchers created a novel fluorescent probe for detecting biological thiols, like cysteine and glutathione, in living cells. This new probe enables selective thiol detection under biological conditions.

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Area of Science:

  • Biochemistry
  • Chemical Biology
  • Molecular Imaging

Background:

  • Biological thiols, such as cysteine and glutathione, play critical roles in cellular redox homeostasis and signaling.
  • Accurate detection and imaging of thiols in biological systems are essential for understanding cellular processes and disease mechanisms.
  • Existing methods for thiol detection may lack selectivity or require harsh conditions, limiting their application in live cells.

Purpose of the Study:

  • To develop and characterize a novel fluorescent probe for the selective detection and imaging of biological thiols.
  • To demonstrate the probe's efficacy in detecting biologically relevant thiols under physiological conditions.
  • To validate the probe's utility for visualizing thiol distribution in living human cells.

Main Methods:

  • Synthesis of a new fluorescent probe by modifying the 2,4-dinitrobenzenesulfonyl group with rhodamine 110.
  • Evaluation of the probe's selectivity for thiol species (e.g., cysteine, glutathione) in solution.
  • Application of the probe for fluorescence imaging of thiol species in living human cells.

Main Results:

  • The developed fluorescent probe demonstrated high selectivity for biological thiol species.
  • The probe successfully detected thiols under biologically relevant conditions.
  • In situ imaging of thiol species within living human cells was achieved using the novel probe.

Conclusions:

  • A new rhodamine 110-based fluorescent probe has been successfully synthesized and validated for biological thiol detection.
  • The probe offers a selective and sensitive method for quantifying and imaging thiols in complex biological environments.
  • This tool has significant potential for advancing research in cellular redox biology and diagnostics.