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Updated: Jul 6, 2026
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
Published on: November 26, 2015
[Analysis of human normal prostate cells membrane proteins by shotgun-MS coupled with laser capture microdissection]
1Key Laboratory of Pathobiology, Ministry of Education, Basic Medical School, Jilin University, Changchun 130021, China.
Objective:
To analyze of membrane proteins of human normal prostate epithelial cells.
Methods:
Laser capture microdissection (LCM) technique was utilized to obtain the epithelial cells of human normal prostate. Shotgun-MS was used to generate protein profiles in the epithelial cells of human normal prostate.
Results:
LCM technique successfully separated the normal prostate epithelial cells with homogeneity more than 95%. Under a stringent filter condition (charge +1, Xcorr >/= 1.9; charge +2, Xcorr >/= 2.2; charge +3, Xcorr >/= 3.75; DelCN >/= 0.1), 1164 proteins were identified in the human normal prostate cells, of which 799 had a gene ontology annotation (GOA) indicating a cellular component, others have no GOA terms. Among the GOA terms, 377 (49.15%) were known membrane proteins or membrane associated proteins. In addition to the proteins known to be associated with the membrane, a significant number of novel proteins had also been identified, including several hypothetical proteins and cDNA sequences.
Conclusion:
Shotgun-MS technique coupled with LCM effectively analyzes the proteins of human normal prostate cells, thus helping perfect the complete protein profiles of human normal prostate cells.
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