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[Ocular toxoplasmosis. Comparison between two biological methods to study aqueous humor]
H Pelloux1, M Mouillon, J P Romanet
1Département de Parasitologie, Mycologie médicale et moléculaire, Hôpital A. Michallon, Grenoble.
Presse Medicale (Paris, France : 1983)
|October 26, 1991
Summary
Diagnosing ocular toxoplasmosis is challenging. This study found that evaluating the Desmonts
Area of Science:
- Ophthalmology
- Infectious Diseases
- Immunology
Background:
- Ocular toxoplasmosis diagnosis is complex, often necessitating antibody screening in aqueous humor.
- Anti-Toxoplasma gondii antibodies are crucial for confirming intraocular infection.
Purpose of the Study:
- To evaluate the efficacy of ELISA and indirect immunofluorescence for diagnosing ocular toxoplasmosis.
- To determine Desmonts's C coefficient and sera/aqueous humor densities ratio using ELISA.
Main Methods:
- ELISA and indirect immunofluorescence techniques were employed.
- Investigated 30 controls and 5 patients with suspected ocular toxoplasmosis.
- Calculated Desmonts's C coefficient and sera/aqueous humor densities ratio.
Main Results:
- No false positives were observed in chorioretinitis-free controls.
- Negative serum antibody levels correlated with the absence of ocular toxoplasmosis.
- ELISA-based C coefficient evaluation enhanced diagnostic sensitivity, dependent on ocular inflammation levels.
Conclusions:
- ELISA is a valuable tool for diagnosing ocular toxoplasmosis.
- Evaluating the C coefficient improves diagnostic accuracy for ocular toxoplasmosis.
- Sensitivity is influenced by ocular inflammation extent.