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Updated: Jul 6, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Lectin microarrays for glycoprotein analysis.

Kanoelani T Pilobello¹, Lara K Mahal

¹Department of Chemistry and Biochemistry, University of Texas at Austin, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 28, 2008

Summary

This summary is machine-generated.

Glycosylation, a common protein modification, impacts various biological processes. A new lectin microarray offers a high-throughput method for analyzing these complex glycan structures.

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A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

Published on: October 1, 2009

Area of Science:

Biochemistry
Molecular Biology
Glycobiology

Background:

Glycosylation is a prevalent posttranslational modification, essential for over half of all proteins, creating glycoproteins.
Aberrant glycosylation is implicated in critical biological events such as tumor metastasis, cell signaling, and inflammatory responses.
The intricate nature of glycans and limited rapid analytical tools hinder the functional study of glycosylation.

Purpose of the Study:

To develop a high-throughput analytical tool for glycan profiling.
To overcome the limitations of existing methods for studying glycosylation.

Main Methods:

Development of a lectin microarray platform.
Utilizing standard microarray printing and scanning technologies.
Employing lectins, which are proteins that bind carbohydrates, for glycan detection.

Main Results:

A novel lectin microarray for high-throughput glycosylation analysis has been successfully created.
This method simplifies the complex process of glycan profiling.
The technique leverages established microarray technology for efficient data acquisition.

Conclusions:

The lectin microarray provides a powerful and accessible tool for advancing glycan research.
This technology facilitates a deeper understanding of glycan function in biological systems.
The developed method addresses the need for rapid and efficient analysis of glycosylation patterns.