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Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
Published on: April 20, 2018
Activity-based protein profiling: from enzyme chemistry to proteomic chemistry
Benjamin F Cravatt1, Aaron T Wright, John W Kozarich
1The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA. cravatt@scripps.edu
Annual Review of Biochemistry
|March 28, 2008
Summary
Activity-based protein profiling (ABPP) is a chemical proteomic method that identifies enzyme functions in biological systems. This review covers ABPP technology, its applications, and biological discoveries made using this powerful technique.
Area of Science:
- Proteomics
- Chemical Biology
- Enzymology
Background:
- Genome sequencing provides extensive protein data, but functional assignment remains a challenge.
- Understanding protein function is crucial for advancing biological and medical research.
- Current methods for functional proteomic analysis require further development.
Purpose of the Study:
- To review the fundamental technology of Activity-based Protein Profiling (ABPP).
- To outline the enzyme classes amenable to ABPP analysis.
- To highlight significant biological discoveries enabled by ABPP.
Main Methods:
- Activity-based Protein Profiling (ABPP) utilizes chemical probes to label active enzymes.
- ABPP enables direct assessment of enzyme function within native biological contexts.
- High-throughput screening and mass spectrometry are key components of ABPP.
Main Results:
- ABPP has successfully profiled diverse enzyme classes across various organisms.
- The technique has revealed novel insights into enzyme function and regulation.
- Numerous biological discoveries have been attributed to the application of ABPP.
Conclusions:
- Activity-based Protein Profiling (ABPP) is a versatile and powerful chemical proteomic strategy.
- ABPP significantly advances the characterization of enzyme function on a global scale.
- The continued application of ABPP promises further breakthroughs in understanding biological systems.
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