Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 6, 2026

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
08:24

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Published on: November 11, 2022

Developmentally acquired PKA localisation in mouse oocytes and embryos.

Rachel J Webb1, Lorna Tinworth, Geraint M H Thomas

  • 1Department of Physiology, University College London, Gower Street, London, WC1E 6BT, UK. r.webb@sgul.ac.uk

Developmental Biology
|March 28, 2008
PubMed
Summary

Protein Kinase A (PKA) localization changes dynamically during oocyte and early embryo development, associating with mitochondria as meiotic competence is acquired. This dynamic PKA localization is lost after fertilization, indicating regulated signaling crucial for development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Relaxor behavior in rocksalt cation-ordered material induced by (anti)ferroelectric phase competition.

Nature communications·2026
Same author

Chromatin- and actin-mediated mitochondrial streaming leads to patterning of mitochondrial distribution in oocytes.

Nature communications·2026
Same author

Contemporary Operator Procedural Volumes and Outcomes for TAVR and MTEER in the US.

JAMA cardiology·2026
Same author

Colossal and tunable dielectric tunability in domain-engineered barium strontium titanate.

Nature communications·2025
Same author

Redox-dependent binding and conformational equilibria govern the fluorescence decay of NAD(P)H in living cells.

FEBS letters·2025
Same author

Strain-induced lead-free morphotropic phase boundary.

Nature communications·2025

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Molecular Signaling

Background:

  • A-Kinase Anchoring Proteins (AKAPs) localize Protein Kinase A (PKA) to coordinate cAMP-mediated signaling.
  • The cAMP-PKA pathway regulates oocyte meiotic arrest and resumption.
  • Spatio-temporal PKA localization is critical for oocyte response to cAMP.

Purpose of the Study:

  • To investigate dynamic changes in PKA localization during oocyte and early embryo development.
  • To determine if PKA localization shifts correlate with acquisition of meiotic competence.
  • To identify potential AKAPs involved in PKA mitochondrial targeting.

Main Methods:

  • Utilized fluorescently-labeled PKA constructs to visualize localization in live oocytes and embryos.
  • Employed RT-PCR and Western blotting to identify candidate AKAPs.

More Related Videos

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
11:13

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment
07:03

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Published on: July 23, 2011

Related Experiment Videos

Last Updated: Jul 6, 2026

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
08:24

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Published on: November 11, 2022

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
11:13

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment
07:03

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Published on: July 23, 2011

  • Observed PKA localization during different developmental stages: meiotically incompetent oocytes, GV-stage oocytes, oocyte maturation, and 2-cell stage embryos.

    • Main Results:

    • In meiotically incompetent oocytes, PKA is diffusely cytoplasmic.
    • PKA associates with mitochondria as oocytes acquire meiotic competence and during maturation.
    • PKA's punctate, mitochondrial localization is lost by the 2-cell stage post-fertilization.
    • AKAP1 and D-AKAP2 were identified as potential mitochondrial AKAPs.

    Conclusions:

    • PKA localization undergoes dynamic regulation throughout oocyte and early embryo development.
    • Mitochondrial association of PKA appears linked to meiotic competence acquisition.
    • The loss of PKA localization post-fertilization suggests a shift in signaling pathways during early embryogenesis.