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Comparison of cellular ribonucleoprotein complexes associated with the APOBEC3F and APOBEC3G antiviral proteins

Sarah Gallois-Montbrun1, Rebecca K Holmes, Chad M Swanson

  • 1Department of Infectious Diseases, King's College London School of Medicine, London SE1 9RT, United Kingdom.

Journal of Virology
|March 28, 2008
PubMed

Insights

Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F (APOBEC3F) and A3G proteins, crucial for retroelement inhibition, exhibit distinct ribonucleoprotein complex compositions, impacting their functions.

Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F (APOBEC3F) and A3G are key antiviral proteins.
  • These proteins share significant sequence identity and inhibit retroelements.

Purpose of the Study:

  • To comparatively analyze the protein and RNA composition of APOBEC3F (A3F) and A3G-associated ribonucleoprotein complexes (RNPs).
  • To understand the structural basis for potential functional differences between A3F and A3G.

Main Methods:

  • Comparative analysis of protein and RNA components within A3F and A3G RNPs.
  • Assessment of RNP association with cytoplasmic microdomains (processing bodies, stress granules).
  • Evaluation of RNP resistance to RNase digestion and identification of associated RNAs.

Main Results:

  • Both A3F and A3G associate with cytoplasmic RNPs and accumulate in processing bodies and stress granules.
  • A3F RNPs show greater resistance to RNase digestion compared to A3G RNPs.
  • A3F RNPs lack Ro60, La autoantigens, Y RNAs, and 7SL RNA, but contain Alu RNA, similar to A3G RNPs.

Conclusions:

  • Subtle differences exist in the protein and RNA composition of A3F and A3G RNPs.
  • These compositional differences likely contribute to the distinct functional properties of A3F and A3G in retroelement restriction.

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