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Updated: Jul 6, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
Published on: August 16, 2016
Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid
Louis P Hromada1, Brian J Nablo, John J Kasianowicz
1University of Maryland, College Park, Department of Mechanical Engineering, College Park, Maryland 20742, USA.
Abstract:
The measurement of single poly(ethylene glycol) (PEG) molecules interacting with individual bilayer lipid membrane-bound ion channels is presented. Measurements were performed within a polymer microfluidic system including an open-well bilayer lipid membrane formation site, integrated Ag/AgCl reference electrodes for on-chip electrical measurements, and multiple microchannels for independent ion channel and analyte delivery. Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed, and measurements of interactions between the membrane-bound ion channels and single PEG molecules are presented.

