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Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

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Updated: Jul 6, 2026

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
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ICPL--isotope-coded protein label.

Josef Kellermann1

  • 1Max-Planck Institute of Biochemistry, Martinsried, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
PubMed
Summary
This summary is machine-generated.

Stable isotope labeling using isotope-coded protein label (ICPL) allows high-throughput proteome profiling. This method accurately quantifies thousands of proteins in complex mixtures for broad proteomic applications.

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Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Stable isotope labeling coupled with mass spectrometry is vital for protein identification and quantification in complex mixtures.
  • Existing methods face limitations in throughput and applicability across diverse sample types.

Purpose of the Study:

  • To introduce and validate a novel high-throughput quantitative proteome profiling method.
  • To demonstrate the broad applicability and accuracy of the isotope-coded protein label (ICPL) technique.

Main Methods:

  • Developed isotope-coded protein label (ICPL) based on stable isotope tagging of free amino groups on intact proteins.
  • Applied ICPL to complex protein mixtures, including tissue and body fluid extracts.
  • Validated compatibility with standard proteomic separation techniques.

Main Results:

  • Achieved high-throughput, global-scale quantitative proteome profiling.
  • Demonstrated highly accurate and reproducible protein quantification.
  • Obtained high sequence coverage, essential for detecting post-translational modifications and protein isoforms.

Conclusions:

  • ICPL is a versatile and robust method for quantitative proteomic analysis.
  • The technique enables accurate quantification and detailed characterization of complex proteomes.
  • ICPL facilitates advancements in identifying protein isoforms and post-translational modifications.