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Related Experiment Video

Updated: Jul 6, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Maize embryogenesis.

Pilar Fontanet¹, Carlos M Vicient

¹Laboratori de Genetica Molecular i Vegetal, Consorci CSIC-IRTA, Spain.

Methods in Molecular Biology (Clifton, N.J.)

|March 29, 2008

Summary

Maize embryo development offers insights into grass embryogenesis due to its unique characteristics and available resources. This study details essential techniques for studying maize embryo development, including in vitro culture and hybridization.

Area of Science:

Plant developmental biology
Grass embryogenesis research
Maize genetics

Background:

Maize embryos have a distinct structure with a scutellum and embryonic axis.
Unlike Arabidopsis, maize embryo cell division planes are less predictable after initial stages.
Maize is an important model organism for studying grass embryogenesis due to genetic resources and economic significance.

Purpose of the Study:

To outline fundamental concepts for studying maize embryo development.
To describe essential techniques for maize embryogenesis research.
To provide a guide for researchers utilizing maize as a model system.

Main Methods:

Greenhouse cultivation of maize plants.
In vitro embryo culture techniques.

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Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

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Published on: February 14, 2020

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

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Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

Somatic embryogenesis protocols.

In situ hybridization methods.

Main Results:

Established protocols for maize cultivation and embryo handling.
Detailed methods for in vitro embryo culture and somatic embryogenesis.
Guidance on applying in situ hybridization for developmental studies.

Conclusions:

Maize is a valuable model for grass embryogenesis research.
Standardized techniques are crucial for reproducible maize embryo studies.
This work provides foundational knowledge for advancing the field.