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Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Gene Conversion02:08

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Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
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Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...

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Genetic Manipulation in &Delta;ku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
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Published on: July 12, 2013

Triplex-mediated gene modification.

Erica B Schleifman1, Joanna Y Chin, Peter M Glazer

  • 1Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
PubMed
Summary
This summary is machine-generated.

This chapter details using triplex-forming molecules for precise gene editing. These DNA-binding agents enable targeted mutagenesis and recombination at specific chromosomal locations.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Gene targeting enables precise genetic modifications.
  • DNA-binding molecules offer routes for site-specific gene alteration.

Purpose of the Study:

  • To describe protocols for designing and utilizing triplex-forming molecules.
  • To facilitate gene modification at specific chromosomal targets.

Main Methods:

  • Design of triplex-forming oligonucleotides and peptide nucleic acids.
  • Identification of target DNA sites.
  • Testing binding affinity and assessing gene modification outcomes.

Main Results:

  • Protocols for creating and applying triplex-forming molecules are provided.
  • Methods for evaluating binding and gene modification efficiency are detailed.

Conclusions:

  • Triplex-forming molecules are effective tools for site-specific gene targeting.
  • This chapter serves as a practical guide for researchers in gene modification.