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Related Experiment Video

Updated: Jul 6, 2026

Stromal Cell Isolation From Hematopoietic Organs
05:27

Stromal Cell Isolation From Hematopoietic Organs

Published on: January 26, 2024

A method to isolate and purify human bone marrow stromal stem cells.

Stan Gronthos1, Andrew C W Zannettino

  • 1Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and Veterinary Science, Hanson Institute, University of Adelaide, SA, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
PubMed
Summary
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The Nagoya University and the University of Adelaide Joint PhD Degree Program in Medicine - A Program of Opportunity.

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Researchers isolated human bone marrow stromal stem cells (BMSSC) using STRO-1 antibody and dual-color fluorescence activated cell sorting. This method enhances purification of colony-forming BMSSC by targeting STRO-1bright and CD106 co-expression.

Area of Science:

  • Stem Cell Biology
  • Immunology
  • Biotechnology

Background:

  • Human bone marrow stromal stem cells (BMSSC) are crucial for regenerative medicine.
  • Existing methods for BMSSC isolation have limitations in purity and efficiency.
  • STRO-1 antibody shows specificity for clonogenic BMSSC.

Purpose of the Study:

  • To develop a highly purified preparation of human bone marrow stromal stem cells (BMSSC).
  • To combine immunoselection techniques for enhanced BMSSC isolation.
  • To identify a reliable method for isolating colony-forming BMSSC.

Main Methods:

  • Initial isolation of STRO-1 antigen-expressing cells using magnetic activated cell sorting (MACS).
  • Secondary purification using dual-color fluorescence activated cell sorting (FACS).

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Last Updated: Jul 6, 2026

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  • Targeting STRO-1bright and CD106 (VCAM-1) co-expression for purification.

    • Main Results:

    • Successful isolation of a minor subpopulation of bone marrow mononuclear cells expressing STRO-1.
    • Further purification of STRO-1bright expressing cells via CD106 co-expression.
    • Generation of highly purified BMSSC preparations containing all colony-forming cells.

    Conclusions:

    • A two-step immunoselection protocol combining MACS and FACS provides highly purified BMSSC.
    • Co-expression of STRO-1bright and CD106 is a reliable marker for colony-forming BMSSC.
    • This method offers an improved approach for isolating clinically relevant BMSSC populations.