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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
09:20

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Published on: March 31, 2016

Adipose-derived stem cells.

John K Fraser1, Min Zhu, Isabella Wulur

  • 1Cytori Therapeutics Inc., San Diego, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
PubMed
Summary
This summary is machine-generated.

Human adipose tissue contains stem cells (ADSCs) with high proliferation and differentiation potential. Liposuction provides a rich source of ADSCs for research, yielding more cells than bone marrow.

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Last Updated: Jul 6, 2026

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07:35

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Published on: March 7, 2015

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Human adipose tissue harbors stem cells with multipotent differentiation capabilities.
  • Adipose tissue-derived stem cells (ADSCs) share similarities with mesenchymal stem cells (MSCs).
  • ADSCs can be sourced from cosmetic liposuction or resected adipose tissue for research purposes.

Purpose of the Study:

  • To describe surgical methods for optimizing ADSC recovery.
  • To detail the enzymatic digestion process for isolating ADSCs from adipose tissue.
  • To highlight the efficiency of ADSC isolation from liposuction aspirates.

Main Methods:

  • Surgical procedures for adipose tissue harvesting.
  • Washing and collagenase digestion of aspirated adipose tissue.
  • Isolation and expansion of ADSCs from the resulting cell population.

Main Results:

  • Liposuction yields large volumes of adipose tissue (approx. 2 L).
  • ADSCs are present at a relatively high frequency within the digested tissue.
  • This method provides a greater yield of stem cells compared to bone marrow extraction without extensive culture expansion.

Conclusions:

  • Adipose tissue is a valuable and accessible source of stem cells for research.
  • Liposuction procedures facilitate efficient recovery of adipose tissue-derived stem cells.
  • ADSCs offer a promising alternative to bone marrow-derived stem cells for regenerative medicine applications.