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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 6, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

BeadArray-based genotyping.

Helen Butler1, Jiannis Ragoussis

  • 1Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
PubMed
Summary
This summary is machine-generated.

Illumina BeadArray technology provides flexible SNP genotyping for complex disease studies. Its GoldenGate assay efficiently analyzes DNA polymorphisms with high accuracy using fluorescent tags.

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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

  • Genomics
  • Biotechnology
  • Molecular Biology

Background:

  • Complex diseases require analysis of numerous single nucleotide polymorphisms (SNPs).
  • Existing technologies face limitations in scalability and flexibility for large-scale SNP interrogation.
  • Linkage and association studies are crucial for understanding complex disease genetics.

Purpose of the Study:

  • To describe the Illumina BeadArray technology and its GoldenGate assay for SNP analysis.
  • To highlight the platform's flexibility for custom and whole-genome SNP panels.
  • To assess the efficiency and reliability of the GoldenGate assay for genetic studies.

Main Methods:

  • Utilizes BeadArray technology with DNA immobilized on beads in random arrays.
  • Employs GoldenGate assay for targeted SNP genotyping.
  • Visualizes SNPs using fluorescent tags to differentiate alleles.

Main Results:

  • The platform is scalable for mid- to high-throughput projects (manual or automated).
  • Requires minimal starting material and tolerates some sample degradation.
  • Assays demonstrate high efficiency, reliability, and high pass rates with excellent concordance levels.

Conclusions:

  • Illumina BeadArray technology, particularly the GoldenGate assay, offers a robust and flexible solution for SNP genotyping.
  • The platform supports diverse applications in genetic research, including complex disease studies.
  • High throughput, efficiency, and reliability make it suitable for large-scale genetic analyses.