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Analysis of geminivirus DNA replication by 2-D gel
1Biological Chemistry, John Innes Centre, Norwich, NR4 7UH, UK.
Abstract:
The technique described was developed for the separation of begomovirus DNA. DNA products resulting from and during geminiviral replication are characterized by the application of strand-specific separation and identification by strand-specific DNA probing of Southern blots. The mapping of the initiation site of complementary-strand DNA synthesis, by this technique is also presented.
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