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Updated: Jul 6, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
Detection of siRNAs and miRNAs
Sakari Kauppinen1, Zoltán Havelda
1Wilhelm Johannsen Center for Functional Genome Research, Department of Cellular and Molecular Medicine, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
Abstract:
Small RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) play crucial roles in establishing general host defense mechanisms against viral infections in plants and the development of disease symptoms. Understanding these fundamental processes requires the sensitive and specific detection of small RNA species. However, because of the small size of miRNAs and siRNAs, their detection is technically demanding. Here, we describe methods for robust and sensitive detection of small RNAs by Northern blot analysis and in situ hybridization.
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