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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Geminivirus: biolistic inoculation and molecular diagnosis
Anésia A Santos1, Lilian H Florentino, Acássia B L Pires
1Departamento de Bioquímica e Biologia Molecular, BIOAGRO- Universidade Federal de Viçosa-36571.000, Viçosa, MG, Brazil.
Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
Summary
Researchers developed a biolistic inoculation method to propagate geminiviruses using DNA from infected plants. This technique aids in studying geminiviruses with single-stranded DNA genomes in various crops.
Area of Science:
- Plant virology
- Molecular biology
- Genomics
Background:
- Geminiviridae is a large family of plant viruses with single-stranded DNA genomes.
- These viruses infect a wide range of important crop species.
- Studying geminiviruses is crucial for agricultural productivity and food security.
Purpose of the Study:
- To describe a novel biolistic inoculation protocol for geminivirus propagation.
- To enable the study of uncloned and non-sap-transmissible geminiviruses.
- To facilitate direct investigation of geminivirus biological properties.
Main Methods:
- Extraction of total DNA from geminivirus-infected plants.
- Biolistic (gene gun) inoculation of extracted DNA into permissive host plants.
- Propagation and analysis of newly introduced geminivirus species.
Main Results:
- Successful propagation of new geminivirus species using the described protocol.
- Demonstration of the protocol's efficacy with total DNA from infected plants.
- Facilitation of direct biological property investigation for challenging geminiviruses.
Conclusions:
- The biolistic inoculation protocol is an effective method for geminivirus propagation.
- This technique overcomes limitations associated with sap-transmission and cloning.
- It provides a valuable tool for advancing geminivirus research and management.
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