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Related Experiment Video

Updated: Jul 6, 2026

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
09:52

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

Noninvasive quantitative imaging of lymph function in mice.

Sunkuk Kwon1, Eva M Sevick-Muraca

  • 1Division of Molecular Imaging, Department of Radiology, Baylor College of Medicine, Houston, TX 77030-3411, USA. kwon@bcm.edu

Lymphatic Research and Biology
|March 29, 2008
PubMed
Summary

This study introduces a novel, noninvasive method for imaging rodent lymphatic function using near-infrared dye. This technique quantifies propulsive lymph flow, crucial for developing new lymph therapeutics.

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Area of Science:

  • Biomedical imaging
  • Lymphatic system research
  • Drug discovery

Background:

  • Noninvasive imaging of propulsive lymphatic function in rodents is essential for lymph therapeutic drug discovery.
  • Previous methods lacked the ability to visualize dynamic lymph propulsion in vivo.
  • A novel near-infrared (NIR) dye approach is presented to overcome these limitations.

Purpose of the Study:

  • To develop and validate a noninvasive method for measuring propulsive lymphatic function in rodents.
  • To enable quantitative assessment of lymph flow dynamics.
  • To provide a tool for evaluating potential lymph therapeutics.

Main Methods:

  • Intradermal injection of indocyanine green (IC-Green), a near-infrared dye, into rodent tails and limbs.

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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

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Related Experiment Videos

Last Updated: Jul 6, 2026

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
09:52

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors
08:39

Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors

Published on: January 7, 2019

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
16:48

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

Published on: August 23, 2007

  • Dynamic imaging of IC-Green trafficking through lymphatic vessels and lymphangions.
  • Analysis of fluorescence intensity to determine lymph flow velocity and frequency.

    • Main Results:

    • Successfully visualized IC-Green transport from injection sites to regional lymph nodes (ischial and axillary).
    • Quantified lymph flow velocity in tail lymph vessels (1.3–3.9 mm/s) and axillary vessels (0.28–1.35 mm/s).
    • Detected pulsatile lymph flow in deep vessels and axillary lymphatics, with varying frequencies.

    Conclusions:

    • The developed method allows for noninvasive and quantitative imaging of propulsive lymph flow in rodents.
    • This technique offers a new platform for investigating lymphatic system function.
    • It holds potential for assessing the efficacy of novel lymph therapeutics.