Heat shock proteins HSP70 and HSP90 in pea seedlings under clinorotation of different duration

We have previously shown an increase in the Hsp70 and Hsp90 level in pea seedlings (cv. Damir) in response to clinorotation. In this work, Western-blot analysis of the Hsp70 and Hsp90 under clinorotation of different duration--from hours to days--was carried out with pea seedlings (cv. Intensive) with more intensive seed germination and seedling growth. Under clinorotation, the higher Hsp70 and Hsp90 amounts in the germinating seeds seem to be caused by some deceleration of their hydrolysis that correlated with common slowing down of seedling growth. Time-dependent changes in response to clinorotation were determined: some temporary increase in the Hsps level with the maximum for the Hsp70 at 2 h of clinorotation and for the Hsp90--at 6 h and their subsequent returning to the control level.