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Related Experiment Videos

Securin' M-phase entry.

Mark J Solomon1, Janet L Burton

  • 1Yale University School of Medicine, Department of Molecular Biophysics and Biochemistry, 333 Cedar Street, New Haven, CT 06520-8024, USA. Mark.Solomon@yale.edu

Nature Cell Biology
|April 2, 2008
PubMed
Summary
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The anaphase-promoting complex (APC) targets cell cycle proteins for degradation. Securin, a substrate, also blocks cyclin B degradation, enabling M-phase entry.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The anaphase-promoting complex (APC) is crucial for cell cycle progression.
  • APC targets key regulators like cyclins and securin for ubiquitination and degradation during M-phase.
  • Securin's role in regulating cyclin degradation is not fully understood.

Purpose of the Study:

  • To elucidate the regulatory mechanism of cyclin B degradation by the APC.
  • To investigate the interplay between securin and cyclin B within the APC pathway.
  • To understand how substrate competition influences M-phase entry.

Main Methods:

  • Ubiquitination assays
  • Degradation assays
  • Analysis of M-phase regulators

Related Experiment Videos

  • Biochemical studies of APC activity

    • Main Results:

    • The APC mediates the degradation of cyclins and securin.
    • Securin directly inhibits the degradation of cyclin B.
    • This substrate competition allows for sufficient cyclin accumulation to initiate M-phase.

    Conclusions:

    • Securin acts as a competitive inhibitor of cyclin B degradation by the APC.
    • This regulatory mechanism ensures timely entry into M-phase.
    • The APC's substrate selectivity is finely tuned for cell cycle control.