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Updated: Jul 6, 2026

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An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis
Published on: October 12, 2016
Materials employed by medical students preparing for subject examinations: supporting collection development
1Library of the Health Sciences-Urbana, University of Illinois at Chicago, 102 Medical Sciences Building, 506 South Mathews Avenue, Urbana, IL 61801, USA. shultz@uic.edu
Journal of the Medical Library Association : JMLA
|April 2, 2008
Summary
No abstract available in PubMed .
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The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

