Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lithographic patterning of conformal thin films on 3D structures using Scaffold-architected Lift-off masks.

Nature communications·2026
Same author

Suspended Germanium-on-Silicon Photonic Integrated Circuits Operating in the Long-Wave Infrared and Their Use for Ethanol Sensing.

ACS photonics·2026
Same author

Conventional Chromatographic Techniques and Biosensors for Mercury Speciation in Seafood: A Systematic Review.

Foods (Basel, Switzerland)·2026
Same author

Continuous Optical Biosensing of IL-8 Cancer Biomarker Using a Multimodal Platform.

Bioengineering (Basel, Switzerland)·2025
Same author

Volatile and non-volatile nano-electromechanical switches fabricated in a CMOS-compatible silicon-on-insulator foundry process.

Microsystems & nanoengineering·2025
Same author

Atmospheric-level carbon dioxide gas sensing using low-loss mid-IR silicon waveguides.

Optics express·2025

Related Experiment Video

Updated: Jul 6, 2026

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
10:59

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

Label-free optical biosensing with slot-waveguides.

Carlos A Barrios1, María José Bañuls, Victoria González-Pedro

  • 1Instituto de Sistemas Optoelectrónicos y Microtecnología, Universidad Politécnica de madrid, ETSI Telecommunicación, Ciudad Universitaria s/n, Madrid, Spain. cbarrios@die.upm.es

Optics Letters
|April 3, 2008
PubMed
Summary

This study presents a label-free biosensor for detecting bovine serum albumin (BSA) and anti-BSA. The integrated silicon nitride/silicon dioxide slot-waveguide microring resonator shows high sensitivity and low detection limits for these biomolecules.

More Related Videos

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

Related Experiment Videos

Last Updated: Jul 6, 2026

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
10:59

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

Area of Science:

  • Photonics
  • Biotechnology
  • Materials Science

Background:

  • Label-free biosensing is crucial for real-time monitoring of biomolecular interactions.
  • Integrated optical devices offer miniaturization and high sensitivity for biosensing applications.
  • Silicon nitride (Si3N4)/silicon dioxide (SiO2) slot-waveguide microring resonators provide a robust platform for photonic sensing.

Purpose of the Study:

  • To demonstrate a novel label-free biosensor utilizing a Si3N4/SiO2 slot-waveguide microring resonator.
  • To quantify the detection capabilities for bovine serum albumin (BSA) and anti-BSA binding events.
  • To assess the sensitivity and detection limits of the integrated biosensor.

Main Methods:

  • Fabrication of an integrated biosensor based on a Si3N4/SiO2 slot-waveguide microring resonator.
  • Monitoring of molecular binding events by measuring resonant wavelength shifts.
  • Varying concentrations of BSA and anti-BSA to determine sensor response.

Main Results:

  • The biosensor successfully detected label-free binding of BSA and anti-BSA.
  • Sensitivities of 1.8 nm/(ng/mm2) for anti-BSA and 3.2 nm/(ng/mm2) for BSA were achieved.
  • Estimated detection limits were 28 pg/mm2 for anti-BSA and 16 pg/mm2 for BSA.

Conclusions:

  • The Si3N4/SiO2 slot-waveguide microring resonator biosensor is effective for label-free biomolecule detection.
  • The demonstrated sensitivity and low detection limits highlight the potential for sensitive biomolecular analysis.
  • Wavelength resolution was identified as the limiting factor for further improving detection limits.