A simple and inexpensive on-column frit fabrication method for fused-silica capillaries for increased capacity and versatility in LC-MS/MS applications
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Updated: Jul 6, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Published on: December 4, 2021
Ling-Chi Wang1, Cindy Yen Okitsu, Harold Kochounian
1Department of Molecular Microbiology and Immunology, USC Norris Cell and Tissue Imaging Core, USC/Norris Comprehensive Cancer Center, Keck School of Medicine at USC, Los Angeles, CA, USA.
Abstract:
A modified sol-gel method for a one-step on-column frit preparation for fused-silica capillaries and its utility for peptide separation in LC-MS/MS is described. This method is inexpensive, reproducible, and does not require specialized equipments. Because the frit fabrication process does not damage polyimide coating, the frit-fabricated column can be tightly connected on-line for high pressure LC. These columns can replace any capillary liquid transfer tubing without any specialized connections up-stream of a spray tip column. Therefore multiple columns with different phases can be connected in series for one- or multiple-dimensional chromatography.
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