Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electron-nuclear double resonance in melanins.

T Sarna, C Mailer, J S Hyde

    Biophysical Journal
    |October 1, 1976
    PubMed
    Summary

    Electron-nuclear double resonance (ENDOR) studies reveal that melanin's proton interactions are sensitive to pH and sample type. These findings demonstrate the feasibility of using ENDOR for further melanin research.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    'Oxygen Level in a Tissue' - What Do Available Measurements Really Report?

    Advances in experimental medicine and biology·2020
    Same author

    Clinical and Statistical Considerations when Assessing Oxygen Levels in Tumors: Illustrative Results from Clinical EPR Oximetry Studies.

    Advances in experimental medicine and biology·2020
    Same author

    Postoperative cognitive dysfunction and mortality following lung transplantation.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
    Same author

    Skeletal Muscle Oxygenation Measured by EPR Oximetry Using a Highly Sensitive Polymer-Encapsulated Paramagnetic Sensor.

    Advances in experimental medicine and biology·2016
    Same author

    Psychosocial Predictors of Mortality Following Lung Transplantation.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
    Same author

    Calculation of dose conversion factors for doses in the fingernails to organ doses at external gamma irradiation in air.

    Radiation measurements·2015

    Area of Science:

    • Biophysics
    • Materials Science
    • Polymer Chemistry

    Background:

    • Melanin, a biopolymer, is known for its heterogeneous structure and radical properties.
    • Understanding the electronic and structural characteristics of melanin radicals is crucial for various applications.

    Purpose of the Study:

    • To investigate the interaction of matrix protons with stable free radicals in melanins using ENDOR.
    • To assess the influence of pH and sample type on melanin's relaxation properties.

    Main Methods:

    • Electron-nuclear double resonance (ENDOR) spectroscopy was employed.
    • Experiments were conducted on "A"- and "B"-type melanins across a range of pH.
    • Melanin samples were incubated in D2O to probe radical accessibility.

    Main Results:

    • Unusually narrow, single ENDOR lines were observed for all melanin samples.
    • ENDOR reduction varied significantly (over a factor of 10) with sample type, indicating pH and sample-dependent relaxation properties.
    • Minimal change in signals after D2O incubation suggests the free radical is protected or buried within the polymer structure.

    Conclusions:

    • Melanin's proton interactions and radical environment are highly sensitive to experimental conditions, particularly pH.
    • The observed ENDOR signals confirm the feasibility of advanced ENDOR studies for detailed melanin characterization.
    • The lack of resolved hyperfine structure supports the view of melanin as a heterogeneous polymer.

    Related Experiment Videos