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Related Experiment Video

Updated: Jul 6, 2026

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

TNF-induced gene expression oscillates in time.

Li Sun¹, Guozhe Yang, Mone Zaidi

¹Department of Medicine, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA.

Biochemical and Biophysical Research Communications

|April 4, 2008

Summary

This summary is machine-generated.

The inflammatory cytokine TNF triggers rapid oscillations in over 5000 genes, revealing gene transcription as a dynamic process. These findings highlight the importance of time-resolved gene expression analysis.

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Area of Science:

Molecular Biology
Genomics
Immunology

Background:

Gene expression is typically considered stable, with few known examples of oscillatory patterns.
Understanding dynamic gene regulation is crucial for deciphering cellular responses to stimuli.

Purpose of the Study:

To investigate the global gene expression patterns in response to the inflammatory cytokine TNF.
To determine if gene expression exhibits oscillatory behavior and characterize its dynamics.

Main Methods:

Utilized microarrays for high-throughput gene expression analysis at 30-minute intervals.
Employed quantitative PCR to confirm observed gene expression oscillations.

Main Results:

TNF induced significant expression changes (>3-fold) in over 5000 genes (15% of the genome).
89% of these genes displayed oscillations with frequencies as low as every 50 minutes.
Oscillations were confirmed to be continuous and not unique to TNF, with RANK-L also inducing oscillations.

Conclusions:

Gene transcription is a highly dynamic process with thousands of genes exhibiting rapid oscillations.
Time-resolved measurements are essential for accurately capturing gene transcription dynamics.
TNF-induced oscillations in gene expression and MAP kinase phosphorylation suggest a cyclical mechanism for transcription factor recruitment.