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NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...

NF-kB-dependent Signaling Pathway

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

The Intrinsic Apoptotic Pathway

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Abnormal Proliferation

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Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...

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Related Experiment Video

Updated: Jul 6, 2026

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

Marasmius oreades substances block NF-kappaB activity through interference with IKK activation pathway.

Roumyana D Petrova¹, Jamal Mahajna, Solomon P Wasser

¹International Center for Cryptogamic Plants and Fungi, Institute of Evolution, University of Haifa, Mt. Carmel, Haifa 31905, Israel.

Molecular Biology Reports

|April 4, 2008

Summary

Marasmius oreades extract fractions inhibit nuclear transcription factor kappa B (NF-kappaB) activation. These compounds block key cancer pathways, reduce cell proliferation, and induce apoptosis in breast cancer cells.

Related Experiment Videos

Last Updated: Jul 6, 2026

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

Area of Science:

Mycology
Molecular Biology
Cancer Research

Background:

Nuclear transcription factor kappa B (NF-kappaB) signaling is crucial in carcinogenesis, promoting cell proliferation, inhibiting apoptosis, and enhancing metastasis.
Marasmius oreades culture liquid extract demonstrated prior inhibitory effects on NF-kappaB activation via IkappaBalpha phosphorylation.

Purpose of the Study:

To investigate the specific mechanism of action of active compounds in Marasmius oreades extract on NF-kappaB activation.
To identify fractions that block the NF-kappaB pathway and assess their anti-cancer effects.

Main Methods:

Liquid chromatography was used to fractionate Marasmius oreades culture liquid extract.
Four fractions were tested for their effects on NF-kappaB activation, IkappaBalpha phosphorylation, p65 nuclear translocation, and IKK pathway activation in MCF7 breast cancer cells.
Cytotoxicity and anti-proliferative potential were evaluated.

Main Results:

All four fractions exhibited anti-proliferative effects on MCF7 cells without cytotoxicity.
Two fractions significantly inhibited IkappaBalpha phosphorylation and NF-kappaB reporter activity.
These active fractions also prevented p65 nuclear translocation, interfered with IKK activation, and induced apoptosis in MCF7 cells.

Conclusions:

Marasmius oreades contains low-molecular-weight bioactive substances with anticancer potential.
These compounds directly block NF-kappaB activation at the IKK level, offering a novel therapeutic strategy.
This study highlights the medicinal value of M. oreades in targeting tumorogenesis.