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Related Experiment Video

Updated: Jul 6, 2026

A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae
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A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

Published on: April 25, 2015

Microarray analysis of Streptococcus pneumoniae gene expression changes to human lung epithelial cells.

Xin-Ming Song1, Wayne Connor, Shakiba Jalal

  • 1Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N5E3, Canada. xinming.song@usask.ca

Canadian Journal of Microbiology
|April 5, 2008
PubMed
Summary

Pathogenic Streptococcus pneumoniae showed significant gene expression changes in lung epithelial cells, unlike avirulent strains. Specific genes were identified that influence bacterial adherence, offering insights into pneumococcal pathogenesis.

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Published on: September 28, 2022

Area of Science:

  • Microbiology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Streptococcus pneumoniae initiates infection in the respiratory tract, interacting with host epithelial cells.
  • Understanding pneumococcal pathogenesis requires investigating bacterial gene expression during host cell interaction.

Purpose of the Study:

  • To compare gene expression profiles of pathogenic and avirulent S. pneumoniae strains upon interaction with human lung epithelial cells.
  • To identify specific genes involved in bacterial adherence and pathogenesis.

Main Methods:

  • Oligonucleotide (oligo)-based microarray analysis was employed to assess gene expression changes.
  • Quantitative real-time PCR (qRT-PCR) was used to validate microarray findings.
  • Knockout mutants were constructed in an avirulent strain to determine gene function.

Main Results:

  • The encapsulated pathogenic strain (serotype 3, strain 82) exhibited differential regulation in numerous functional categories, including pathogenesis and metabolism.
  • The unencapsulated avirulent strain (strain R6) showed minimal gene expression changes, primarily in ribonucleotide biosynthesis.
  • Two genetic loci, SP_2170 (AdcB, zinc ABC transporter) and SP_0157 (hypothetical protein), were identified as crucial for adherence to A549 lung epithelial cells.

Conclusions:

  • Significant divergence in gene expression exists between pathogenic and avirulent S. pneumoniae strains during host cell interaction.
  • Specific identified genes, like SP_2170 and SP_0157, play a role in pneumococcal adherence and contribute to pathogenesis.