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Sequences of four previously undescribed human T-cell receptor beta chain variable genes
T Hansen1, E Qvigstad, K E Lundin
1Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
Tissue Antigens
|September 1, 1991
Abstract:
T-cell receptor beta chain mRNA from 4 human T-lymphocyte clones was reverse transcribed, amplified by PCR, cloned in M13 and sequenced. Four hitherto undescribed variable genes were found. One of them (V beta-IW22) showed the highest homology (74.5%) to V beta 1. Two others (V beta-IW6 and V beta-IW10) belonged to the recently described V beta 21 family. The last gene (V beta-VW114) belonged to the V beta 9 family. However, the latter gene a is pseudogene because of an in-frame stop codon.