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Updated: May 4, 2026
Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
Published on: November 19, 2020
Comparison of embryo development between intracytoplasmic and Piezo-assisted sperm injection after treating mouse
Abdolhossein Shahverdi1, Mansoureh Movahedin1, Mojtaba Rezazadeh Valojerdi1,2
1Department of Anatomy, Medical Science Faculty, Tarbiat Modares University, Tehran, Iran.
Background:
The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development.
Methods:
In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized with or without CaI-treated sperms.
Results:
Piezo-ICSI demonstrated significantly more favorable results, with a fertilization rate of 64% (conventional ICSI: 42%, P<0.001) and a cleavage rate of 73% (conventional ICSI: 58%, P<0.05). When the Piezo-ICSI procedure was performed with CaI-pretreated sperms, the cleavage rate significantly increased (92% vs. 73%, P<0.05). However, the fertilization rate did not change significantly (64% vs. 56%).
Conclusion:
The Piezo-ICSI accompanies with CaI-treated sperms is more efficient than the conventional ICSI method for fertilizing and thus obtaining more mouse embryos.
