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Molecular characterization of a Squalene epoxidase gene in dermatophyte pathogen Trichophyton tonsurans
Kamyar Motavaze1, Zahra Namvar1, Massoud Emami1
1Dept. of Microbiology, School of Basic Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran.
Background:
Trichophyton tonsurans is one of the dermatophyte fungi which invades the skin and hair of human. Several properties of this fungus have been investigated so far. However a few studies were carried out in the field of molecular biology of this fungus. In the present study, we tried to identify the Squalene epoxidase gene which is related to synthesis of ergosterol in this fungus.
Methods:
Pairs of 23 and 24 nucleotides primers were designed from highly conserved regions of the similar genes in other fungi. Mentioned primers were utilized in PCR by using isolated genomic DNA of T. tonsurans whereas the PCR fragments were then sequenced.
Results And Conclusion:
Nucleotides (n = 558) have been sequenced from this new gene which encodes a polypeptide with 186 amino acids. Sequences comparison in gene data banks (NCBI, NIH) for this part of DNA and its deduced amino acid revealed significant homology with members of the eukaryotic Squalene epoxidase.
