Calcium transport and extracellular pH in epithelial membranes

Calcium plays a pivotal role in cell adhesion, ATPase function, and in membrane permeability. The molecular mechanism for these diverse actions include: hormonal factors, activation of intracellular mediators, and physical factors such as ionic mobility and pH. To further examine the effects of one physical factor, pH, we designed studies examining Ca transport in the isolated turtle bladder epithelium. This tissue is a high-resistance epithelium which reabsorbs Na and secretes H+. The turtle has only rudimentary parathyroid tissue, the gland does not respond to a lowered plasma Ca, and cyclic AMP is not a primary intracellular mediator. In a series of in vitro experiments, we examined Ca metabolism under conditions simulating metabolic acidosis and alkalosis. Acidosis markedly inhibited the mucosa-to-serosa Ca flux, while alkalosis stimulated it. The effect of acidosis on the mucosa-to-serosa Ca flux was independent of Na transport. Changing serosal pH had no effect on the serosa-to-mucosa Ca flux or on proton secretion. Total tissue Ca concentration, measured using atomic absorption spectrometry, was identical when the extracellular pH varied from 5.4 to 8.4. When epithelial cells were isolated and Ca uptake was measured over a wide pH range, a linear increase in uptake was seen as pH was increased from 4.4 to 8.4. In separated turtle bladder epithelial cells ATP-dependent Ca transport, in the mitochondrial-rich cells, was 4- to 5-fold higher than activity found in the granular cells. The mitochondrial-rich cells comprise approximately 20% of the total epithelial surface and are thought to be the cells primarily involved in proton secretion.(ABSTRACT TRUNCATED AT 250 WORDS)