Synthesis and secretion of human atrial natriuretic peptide in Saccharomyces cerevisiae

¹Institute of Genetics, Fudan University, Shanghai, PRC.

Science in China. Series B, Chemistry, Life Sciences & Earth Sciences

|December 1, 1991

Abstract:

A chemically synthesized alpha-hANP gene was inserted into plasmid YFD18, which was an expression-secretion vector of yeast. The recombinant then transformed in the yeast Y33. The expression level of yeast transformants was about 700 micrograms ANP/L detected by RIA. More than 99% of expression products were secreted in the culture medium. N-terminal analysis of purified product showed that the first 4 amino acid residues of alpha-hANP were deleted.

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