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Published on: September 11, 2015
Osteoblastic cell behavior on nanostructured metal implants
Laurent Le Guehennec1, Frantz Martin, Marco-Antonio Lopez-Heredia
1INSERM U791, Laboratory for Osteo-Articular & Dental Tissue Engineering, Faculty of dDental Surgery, 44042 Nantes, France.
Aims:
Surface modifications at the nanometric scale may promote protein adsorption, cell adhesion and thus favor the osseointegration of metal implants. The behavior of osteoblastic cells was studied on mirror-polished (Smooth-SS) and nanostructured (Nano-SS) stainless steel surfaces.
Materials & Methods:
The nanostructuration was obtained by anodization. The surfaces were characterized by scanning electron, atomic force microscopy (AFM) and X-ray photoelectron specytoscopy. Osteoblastic cells were cultured on these surfaces.
Results & Discussion:
AFM showed a regular array of pores on the Nano-SS. Osteoblastic cells spread more rapidly on Nano-SS surfaces than on Smooth-SS surfaces. Cell viability was similar on Smooth-SS and Nano-SS. Alkaline phosphatase activity increased with culture time whatever the substrate but was better enhanced at 21 days on the Nano-SS than on the Smooth-SS.
Conclusion:
This study shows that the nanostructuration of metal implants may improve the adhesion and differentiation of osteoblastic cells.
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