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Related Experiment Video

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The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

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Published on: October 3, 2017

Multiple roles for Med12 in vertebrate endoderm development.

Chong Hyun Shin¹, Won-Suk Chung, Sung-Kook Hong

¹Department of Biochemistry and Biophysics, Liver Center, University of California, San Francisco, CA 94158, USA. chong.shin@ucsf.edu

Developmental Biology

|April 9, 2008

Summary

Mutations in the shiri gene disrupt zebrafish endoderm development, affecting liver and pancreas formation. This is partly due to reduced expression of the hairy-related gene, her5, highlighting its role in organogenesis.

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Generation of Naïve Blastoderm Explants from Zebrafish Embryos

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Generation of Naïve Blastoderm Explants from Zebrafish Embryos

Generation of Naïve Blastoderm Explants from Zebrafish Embryos

Published on: July 30, 2021

Area of Science:

Developmental Biology
Genetics
Molecular Biology

Background:

The endoderm forms the digestive tract and associated organs like the liver and pancreas.
Understanding endodermal cell differentiation into specific cell types is crucial.

Purpose of the Study:

To investigate genes regulating endodermal organ development using zebrafish.
To identify the molecular mechanisms underlying endodermal organ defects.

Main Methods:

Forward genetic screen in zebrafish to identify mutations affecting endodermal organ development.
Phenotypic analysis of mutant embryos, including gene expression studies (her5).
Positional cloning to identify the mutated gene (shiri) and molecular characterization of its function.

Main Results:

Mutations at the shiri locus cause defects in liver and pancreas development in zebrafish.
These defects are linked to reduced endodermal expression of her5 during gastrulation.
shiri encodes Med12, a subunit of the transcriptional Mediator complex.
Med12 influences Casanova/Sox32's ability to induce sox17 expression.

Conclusions:

Med12 plays a significant role in vertebrate endoderm development.
her5 is important for the development of endodermal organs like the liver and pancreas.
Defects in Med12 may explain craniofacial and digestive defects in human syndromes.