Related Experiment Video
Updated: Jul 6, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Differential control of the PcoI/PcoR quorum-sensing system in Pseudomonas fluorescens 2P24 by sigma factor RpoS and
Qing Yan1, Xiao-Gang Wu, Hai-Lei Wei
1Department of Plant Pathology, China Agricultural University, Beijing 100094, China.
Abstract:
A quorum-sensing (QS) locus PcoI/PcoR had been identified previously in the biological control bacterium Pseudomonas fluorescens 2P24. In this paper, we investigated the upstream regulators that influence the transcription of the N-acyl homoserine lactone (AHL) biosynthase gene pcoI using a chromosomal pcoIColon, two colonslacZ fusion reporter strain. Stationary sigma factor RpoS was identified as a negative regulator of QS system using a random mini-Tn5 mutant procedure. Furthermore, deletion mutagenesis and complementation experiments demonstrated that the two-component system GacS/GacA positively regulated the QS system by upregulating pcoI transcription. However, compared with the gacA or gacS mutant, introduction of a second mutation of rpoS in the gacA(-) or gacS(-) background did not lead to further change in the transcriptional expression of the pcoI gene or the synthesis of AHL. Our results suggest that in strain 2P24, RpoS could only play its negative regulatory role on the pcoI gene under a functional GacS/GacA system background.
More Related Videos
11:31Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
Published on: June 30, 2016
06:30Using Microtiter Dish Radiolabeling for Multiple In Vivo Measurements Of Escherichia coli (p)ppGpp Followed by Thin Layer Chromatography
Published on: June 4, 2019
Related Concept Videos
Global Regulatory Systems
Gene Regulation in Microbial Communities: Quorum Sensing
Regulation of Bacterial Virulence
Bacterial Signaling
Stringent Response in E. coli
Other Stress Responses in Bacteria