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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jul 6, 2026

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

Published on: April 11, 2021

A focused microarray to study human mitochondrial and nuclear gene expression.

Joachim G Voss¹, Raghavan Raju, Carolea Logun

¹Neuromuscular Diseases Section, National Institute of Neurological Diseases and Stroke, NIH, Bethesda, MD, USA. vossj@u.washington.edu

Biological Research for Nursing

|April 10, 2008

Summary

This summary is machine-generated.

Researchers developed the huMITOchip, a novel microarray for studying human mitochondrial and nuclear gene expression. This tool accurately measures mitochondrial gene expression and shows high concordance with existing arrays.

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Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Gene expression analysis is crucial for understanding health and disease.
Existing microarrays primarily focus on nuclear genes, with limited coverage of the mitochondrial genome.
Mitochondrial dysfunction is implicated in numerous pathologies.

Purpose of the Study:

To develop and validate a focused microarray (huMITOchip) for comprehensive analysis of human mitochondrial and nuclear gene expression.
To assess the utility of the huMITOchip in detecting gene expression changes in response to stimuli.
To provide a tool for investigating mitochondrial gene alterations in various biological contexts.

Main Methods:

Design and implementation of the huMITOchip with 4,774 probe sets, including 51 probes for 37 mitochondrial genes.
Validation using an in vitro system with CCL136 muscle cells stimulated with interferon gamma (IFN-gamma).
Comparative analysis against the Affymetrix U133 plus 2.0 array.

Main Results:

The huMITOchip successfully interrogated 37 mitochondrial genes with expression levels ranging from 0.1 to 3,182.
Excellent correlation (Pearson's r = 0.98) was observed between huMITOchip and the Affymetrix U133 plus 2.0 array.
High concordance in identifying differentially expressed genes (at least 17 probe sets) in response to IFN-gamma was achieved.

Conclusions:

The huMITOchip represents the first focused oligonucleotide microarray specifically designed to include genes of the mitochondrial genome.
This novel tool enables accurate assessment of both nuclear and mitochondrial gene expression.
The huMITOchip is a validated platform for research into gene expression alterations in health and disease, particularly those involving mitochondrial function.