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Related Experiment Videos

Computer-assisted hydrodynamic gene delivery.

Takeshi Suda1, Kieko Suda, Dexi Liu

  • 1Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.

Molecular Therapy : the Journal of the American Society of Gene Therapy
|April 10, 2008
PubMed
Summary
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A new computer-controlled injection device enables precise hydrodynamic delivery of DNA and RNA in large animals, advancing in vivo gene delivery and potential gene therapy applications.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Gene Therapy

Background:

  • Hydrodynamic delivery enables DNA and RNA transfer into parenchyma cells via intravascular injection.
  • Current methods are effective in rodents but challenging in large animals due to manual control limitations for precise pressure regulation.

Purpose of the Study:

  • To develop a computer-controlled injection device for reliable hydrodynamic gene delivery in large animals.
  • To overcome the limitations of manual control in achieving consistent hydrodynamic pressure in targeted tissues.

Main Methods:

  • Development of a computer-controlled injection device regulating pressure in real-time.
  • Utilized mouse liver as a model for continuous and serial injections at programmed pressures.
  • Tested gene delivery in rat kidney and muscle cells using plasmids and adenoviral vectors.

Related Experiment Videos

  • Applied image-guided catheterization with the device for gene delivery in pigs.

    • Main Results:

    • The computer-controlled device demonstrated gene delivery efficiency comparable to conventional methods in mice.
    • Successful gene delivery was achieved in rat kidney and muscle cells.
    • Demonstrated effective gene delivery to liver and kidney in pigs, indicating large animal applicability.

    Conclusions:

    • The developed computer-controlled injection device offers a reliable solution for hydrodynamic gene delivery in large animals.
    • This technology significantly advances in vivo gene delivery research.
    • Presents promising potential for future clinical applications in human gene therapy.