Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cyclic mechanical preconditioning improves engineered muscle contraction.

Du Geon Moon1, George Christ, Joel D Stitzel

  • 1Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA.

Tissue Engineering. Part A
|April 11, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling.

Drug discovery today·2016
Same author

A tunable hydrogel system for long-term release of cell-secreted cytokines and bioprinted in situ wound cell delivery.

Journal of biomedical materials research. Part B, Applied biomaterials·2016
Same author

Re: A Tumor Mitochondria Vaccine Protects against Experimental Renal Cell Carcinoma.

The Journal of urology·2016
Same author

Re: Endogenous Formaldehyde is a Hematopoietic Stem Cell Genotoxin and Metabolic Carcinogen.

The Journal of urology·2016
Same author

Re: Sphaeropsidin A Shows Promising Activity against Drug-Resistant Cancer Cells by Targeting Regulatory Volume Increase.

The Journal of urology·2016
Same author

Re: Sex Hormone-Dependent tRNA Halves Enhance Cell Proliferation in Breast and Prostate Cancers.

The Journal of urology·2016
Same journal

Hydrogel-Encapsulated Primed MSCs Enhance Regeneration in Full-Thickness Porcine Burn Wounds.

Tissue engineering. Part A·2026
Same journal

Unidirectional Porous Carbonate Apatite Fabricated by Gelatin-Based Freeze Casting for Bone Regeneration.

Tissue engineering. Part A·2026
Same journal

Regenerative Nanoscaffolds for Chronic Tympanic Membrane Perforation: From Bench to Clinical Translation.

Tissue engineering. Part A·2026
Same journal

Impact of IFN-γ-Pretreated Umbilical Cord Mesenchymal Stem Cells Implanted in Mesh on Pelvic Organ Prolapse.

Tissue engineering. Part A·2026
Same journal

The Driving Force of Hierarchical Collagen Fiber Formation: A Review of Tendon, Ligament, and Meniscus Mechanobiology.

Tissue engineering. Part A·2026
Same journal

A Nondestructive Raman Spectral Method for Temporal Tracking of Articular Cartilage Maturation.

Tissue engineering. Part A·2026
See all related articles

Engineered skeletal muscle shows improved function after in vitro preconditioning. This novel method enhances contractility in tissue-engineered muscle for reconstructive applications.

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Clinically relevant functional skeletal muscle tissue engineering is challenging.
  • Existing methods struggle to achieve sufficient contractility for reconstructive surgery.

Purpose of the Study:

  • To develop and evaluate an in vitro preconditioning protocol to enhance engineered skeletal muscle contractility.
  • To assess the functional recovery of engineered muscle constructs after in vivo implantation.

Main Methods:

  • Human muscle precursor cells (MPCs) were seeded onto collagen scaffolds.
  • Constructs underwent cyclic strain in a computer-controlled bioreactor (preconditioning) or static culture.
  • Preconditioned constructs were implanted into athymic mice.

Related Experiment Videos

Main Results:

  • Bioreactor preconditioning yielded viable, unidirectionally oriented muscle constructs within 5 days.
  • Engineered muscle generated contractile responses after 3 weeks of bioreactor conditioning.
  • Implanted constructs preconditioned for 1 week showed tetanic and twitch responses, achieving 1% and 10% of native muscle force, respectively.

Conclusions:

  • In vitro bioreactor preconditioning significantly improves the functional contractility of engineered skeletal muscle.
  • This protocol represents a significant advancement for skeletal muscle tissue engineering and regenerative medicine.
  • The generated force is the largest reported for engineered skeletal muscle on an acellular scaffold.