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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jul 6, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Exploring the uremic toxins using proteomic technologies.

Eric Schiffer¹, Harald Mischak, Raymond C Vanholder

¹Mosaiques Diagnostics & Therapeutics, Hanover, Germany.

Contributions to Nephrology

|April 11, 2008

Summary

This summary is machine-generated.

Proteomics, including capillary electrophoresis coupled to mass spectrometry (CE-MS), offers advanced methods for identifying uremic toxins. These novel biomarkers are crucial for understanding kidney failure and developing new diagnostics.

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Last Updated: Jul 6, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

Biochemistry
Nephrology
Analytical Chemistry

Background:

Kidney failure causes uremic syndrome due to the accumulation of toxic substances normally cleared by the kidneys.
Polypeptides are a significant, heterogeneous group of these uremic toxins.
Identifying and characterizing these toxins is vital for understanding disease progression.

Purpose of the Study:

To provide an overview of proteomic technologies for detecting uremic toxins.
To highlight the capabilities of capillary electrophoresis coupled to mass spectrometry (CE-MS) in this field.
To discuss the application of proteomics in identifying novel biomarkers for renal diseases.

Main Methods:

Proteomic analysis, including classical techniques like 2D gel electrophoresis and mass spectrometry for larger polypeptides (>10 kDa).
Capillary electrophoresis coupled to mass spectrometry (CE-MS) for analyzing smaller polypeptides (1-10 kDa), capable of analyzing up to 1,400 compounds.
Review of proteomic applications in biomarker discovery for renal diseases.

Main Results:

Proteomic technologies are effective tools for detecting a wide range of uremic toxins.
CE-MS is a rapid and powerful method for analyzing medium-sized polypeptides in uremic samples.
Proteomic approaches have successfully identified novel biomarkers associated with renal diseases.

Conclusions:

Proteomics offers promising avenues for identifying new uremic toxins and biomarkers.
CE-MS is particularly valuable for the analysis of medium-sized polypeptides in uremic syndrome.
These advancements in proteomic analysis are crucial for improving the diagnosis and understanding of kidney failure.